The current investigation objective was to fabricate osmotic tablets embedded with double walled microspheres of esomeprazole for the treatment of peptic ulcer. The microspheres were prepared by an ionotropic gelation technique using calcium chloride(5%) as cross-linking agent, sodium alginate, gelatin and pectin as mucoadhesive polymers. Total six batches of microspheres were prepared among this F1-F3 contains gelatin as a mucoadhesive polymer together sodium alginate that was added to produce calcium- alginate beads in an ionotropic gelation also act as a second mucoadhesive polymer. The next set of microspheres from F4-F6 contains pectin as first and sodium alginate as second polymer. The formulated microspheres were evaluated based on the parameters- particle size, percentage yield (%), drug incorporation efficiency (%), swelling index (%), drug content (%)and in vitro drug release (%). The best batches of microspheres were selected for fabrication of osmotic tablets by adding osmotic agents and finally polishing with coating solution containing pore former and plasticizer makes thecontrolled porosity osmotic pump tablets. The data obtained after evaluation of the tablets suggest that a micro particulate system can be successfully designed for sustained delivery of esomeprazole and to improve its bioavailability.