For the present study, the presmud and distillery industry wastes were collected at M/S Rajshree Sugars and Chemicals Limited, Varadaraj Nagar, Theni district, Tamilnadu, India, used sterile polythene container and brought to the laboratory to analyze the physical and chemical properties by following the standard method of APHA (2005). The physical and chemical properties of the pressmud were analysed. The physical and chemical properties of the spent wash were also analysed. The biocompost preparation with sugar and distillery industry wastes was optimized using selected microbial species. The In silico study indicates that the Metabolic pathway information of Bacillus circulans and Pseudomonas aeruginosa which was identified from gene/protein sequences analysis have a potential significance in the Biodegradation of sugar and Distillery industry wastes for Biocompost preparation.