Isolation of Methionine - Producing Streptomyces Species from South Indian Soil

International Journal of Novel Trends in Pharmaceutical Sciences,2012,2,1,16-19.
Published:February 2012
Type:Research Article
Authors:
Author(s) affiliations:

Sabitha. R1,*, Abinaya. S2, Nikhil Kumar Sakle3, Karthik Raja. M 4

1Department of Pharmaceutical Biotechnology, Jagan’s College of Pharmacy, Nellore, Andhra Pradesh, India.

2School of Biological Siences, Vellore Institute of Technology University, Vellore, Andhra Pradesh, India.

3Department of Pharmacology,Y.B Chavan College of Pharmacy, Aurangabad, Andhra Pradesh, India.

4Department of Pharmaceutics, S.Chavan College of Pharmacy, Nellore, Andhra Pradesh, India.

Abstract:

Actinomycetes isolated from soil were investigated for methionine production, and an isolate, designated SP-05, was recovered. Taxonomic studies showed that the strain has good growth, sporulation and black pigment production on most media, utilized various sugars, reduced nitrates and degraded tyrosine. The aerial mycelia have spore chains with open loops, and therefore belong to retinaculum apertum group. Presence of LL diaminopimelic acid on the cell wall of isolate SP-05 also suggested that the strain belongs to the genus Streptomyces. The organism was found to produce 3.72 mg/ml methionine in culture broth. Sucrose and ammonium chloride at concentrations of 8.0 and 6.0% (w/v) respectively, in the culture medium, enhanced methionine production.

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Effect of sucrose on methionine production.