The present study was designed to evaluate the antidepressant activity of Valeriana jatamansi (Fam. Valerianaceae) roots. The dichloromethane extract was subjected to antidepressant activity evaluation on lacca mice using forced swimming test. The standard and test extract were administered per oral route. Dichloromethane extract of the roots exhibited significant dose dependent antidepressant activity at 400 mg/kg, comparable to that of standard imipramine (12.5 mg/kg).